Mini Review Acetaminophen Toxicity and 5 Oxoproline (Pyroglutamic Acid): A Tale of Two Cycles, One an ATP Depleting Futile Cycle Yes there is good evidence that acetaminophen (the active ingredient in Tylenol) can deplete glutathione under certain conditions. But whether that matters depends on dose, duration, and individual factors. Glutathione: Master Antioxidant, Reducing Oxidative Stress, and Detoxification Glutathione, Alcohol and drugs Electrochemical evidences in oxidation of acetaminophen in the presence of glutathione and N acetylcysteine Chemical Communications (RSC Publishing)