View of Acetaminophen, the missing anion gap in the patient with metabolic acidosis The Southwest journal of medicine Yes there is good evidence that acetaminophen (the active ingredient in Tylenol) can deplete glutathione under certain conditions. But whether that matters depends on dose, duration, and individual factors. Oxidative stress during acetaminophen hepatotoxicity: Sources, pathophysiological role and therapeutic potential ScienceDirect PDF) A review of the evidence concerning hepatic glutathione depletion and susceptibility to liver toxicity after paracetamol overdose Acetaminophen metabolism