Does Acetaminophen Deplete Glutathione? Understanding the Connection Cymbiotika CYMBIOTIKA The Hidden Impact of Acetaminophen Well Rooted PGC 1 Induces Susceptibility To Acetaminophen Driven Acute Liver Failure Scientific Reports Recurrent Pyroglutamic Acidosis Related to Therapeutic Acetaminophen ScienceDirect Yes there is good evidence that acetaminophen (the active ingredient in Tylenol) can deplete glutathione under certain conditions. But whether that matters depends on dose, duration, and individual factors.