L Carnitine in Mitochondria Encyclopedia MDPI Carnitine, Acylcarnitines and Beta Oxidation Role of L carnitine in the transportation of long chain fatty acid across the mitochondria membrane. Activation and Transportation of Fatty Acids to the Mitochondria via the Carnitine Shuttle with Animation Animations PharmaXChange.info Short Note On Carnitine Shuttle: Fatty acids are oxidized in mitochondria, and synthesized in the cytoplasm. Long chain fatty acids must form an active intermediate, fatty acyl CoA, before being oxidized inside mitochondria. Catherine